THE GROWTH OF PIMPINELLA ALPINA HOST CALLUS AT VARIOUS TREATMENTS OF PLANT GROWTH REGULATOR CONCENTRATIONS OF NAA. 2,4 D AND ITS COMBINATION WITH BAP
Keywords:
Purwaceng, Pimpinella alpina, Callus Induction, Tissue Culture, Murashige Skoog
Abstract
Purwaceng (Pimpinella alpina) is an herbal plant that has aphrodisiac, diuretic, and tonic properties. The imbalance between the growth and the exploitation of this plant makes it included in Appendix I (endangered), so its conservation will be crucial. One method of the conservation of this plant is tissue culture. This method is considered one way to induct the callus. The research objective is to find the effect of various treatments of plant growth regulator concentrations of NAA. 2,4 D and its combination with BAP on the growth of purwoceng callus. Concentration of plant growth regulator NAA (0.5 mg L-1, 1 mg L-1, 2 mg L-1), 2,4-D (0.4 mg L-1, 0.8 mg L-1, 1.2 mg L-1 ), a combination of NAA with BAP (0.5 mg L-1 NAA: 1 mg L-1 BAP, 1 mg L-1 NAA: 2 mg L-1 BAP, mg L-1 NAA: 3 mg L-1 BAP) and a combination of 2, 4- D with BAP (1 mg LL 2,4-D : 0.5 mg L BAP, 1 mg L-1 2,4-D : 1 mg L-1 BAP, 2 mg L-1 2,4-D : 1 mg L-1 BAP) into Murashige Skoog medium. Then purwaceng leaf explants were planted on the medium until the growth was seen. This research shows that the plant growth regulator concentration has a different influence on the growth of callus that is recognized by the day of callus induction, colour and callus texture. MS media with growth regulator 1.2 mg L-1 2,4-D can grow callus faster (17 days) with friable texture.
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References
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Syahid S.F., Kristina N.N., and Seswita D. 2010. Effect of media composition on callus growth and tannin content of Dutch teak leaves (Guazuma ulmifolia Lamk) in vitro.
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Widodo P., Proklamasiningsih E., Sudiana E., Yani E., Budisantoso I., and Herawati W. 2018. Distribution of Purwoceng (Pimpinella Pruatjan Molkenb.) Past and Present. Seminar Proceedings National dan Call for Papers. (November): 376-383.
Widyawati G. 2010. Effect of variations in NAA and BAP concentrations on callus induction of Jatropha (Jatropha Curcas L.). UNS (Sebelas Maret University).
Zaerr J.B, and Mapes M.O. Action of growth regulators; 231–255 in Tissue culture in forestry, Springer.
Ariningsih, I.S., and Endang A. 2003. Callus Growth and Production of Noni Anthraquinone (Morinda citrifolia, L.) on Murashige-Skoog (MS) Media with the Addition of Ca2+ and Cu2+ Ions. Biopharmaceuticals. 1(2): 39– 43.
Bhatia, S. 2015. Chapter 2 - Plant Tissue Culture, 31–107 in S. Bhatia, K. Sharma, R. Dahiya, dan T. Bera, eds., Modern Applications of Plant
Biotechnology in Pharmaceutical Sciences, Academic Press, Boston.
Darwati I., and Roostika I. 2016. Research Status of Purwoceng (Pimpinella alpina Molk.) in Indonesia. Germplasm Bulletin. 12(1): 9.
Dwiyono E. 2009. Callus Induction of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) Plants with Dark Conditions and 2, 4-D Treatment
(Undergraduate Thesis).
Faramayuda F., Mariani T.S., Elfahmi and Sukrasno. 2021a. Identification of secondary metabolites from callus Orthosiphon aristatus (Blume) miq by thin layer chromatography. Sarhad Journal of Agriculture. 37(3): 1081-1088.
Faramayuda F., Mariani T.S., Elfahmi and Sukrasno. 2021b. Micropropagation and secondary metabolites content of white-purple varieties of Orthosiphon aristatus Blume miq. Pakistan Journal of Biological Sciences. 24(8): 858-867.
Faramayuda F., Mariani T.S., Elfahmi and Sukrasno. 2021c.Chemical compound identification of two varieties cat whiskers (Orthosiphon aristatus Blume Miq) from in vitro culture. Sarhad Journal of Agriculture. 37(4): 1355-1363.
Fatmawati A. 2008. Study of BAP concentration and 2, 4-D on callus induction of Artemisia annua L. In vitro (Thesis). Faculty of Agriculture. Eleven March State University. Surakarta.
Gunawan L.W. 1995. In vitro Plant Culture Techniques, Intermediate Center, Bogor.
Intias S. 2011. Effect of giving various concentrations of 2, 4-d and bap on the formation of callus purwoceng (Pimpinella pruatjan) in vitro. Thesis.
Katuuk J.R.P. 1989. Tissue culture techniques in plant micropropagation. Ministry of Education and Culture Director General of Higher Education, Jakarta.
Kresnawati E. 2006. Effect of growth regulators NAA and kinetin on callus induction from Patchouli (Pogostemon cablin Benth) leaves. Muhammadiyah University of Surakarta.
Lestari, K., Deswiniyanti, N. I., Astarini, I., dan Arpiwi, L. U. H. 2019. Callus and shoot induction of leaf culture Lilium longiflorum with NAA and BAP, Nusantara Bioscience, 11, 162-165.
Maggon R., and Singh B.D. 1995. Promotion of adventitious bud regeneration by ABA in combination with BAP in epicotyl and hypocotyl explants of sweet orange (Citrus sinensis L. Osbeck). Scientia Horticulturae. 63(1-2): 123–128.
Mariani T.S. 2017.Meristem Culture and its Stigmasterol Content of Purwoceng (Pimpinella pruatjan Molk.) for Agricultural Sciences. International Journal of Innovations in Engineering and Technology(IJIET). 250-253.
Meiny. 2017. Effect of Purwoceng Extracts (Pimpinella alpina Molk) on Erectile Function Biomarker Enhancement. PJMHS. 1182-1187.
Pierik R.L.M. 1997. In vitro culture of higher plants. Springer science and business media.
Purwianingsih W., Kusdianti R., and Yuniarti L. 2007. Anatomy of callus from leaf explants of Catharanthus roseous (L). G. Don (Tapak Dara). Journal of the National Seminar on Biotechnology.
Rismayanti H.F. 2010. Effect of chlorox (NaOCl) on surface sterilization for the development of Aglaonema (Donna carmen) seedlings in vitro. Proceedings of Scientific Seminar and Annual Meeting of PEI and PFI XX South Sulawesi Regional Commissariat.
Rusmin D. 2017. Development of Purwoceng (Pimpinella pruatjan Molk.) Cultivation as a Medicinal Plant. Perspective. 16(2): 80-93.
Santoso U., and Nursandi F. 2004.Plant Tissue Culture. University of Muhammadiyah Malang, Press. Poor.
Sari N. 2013. Effect of Addition of Various Combinations of Concentrations of 2, 4-Dichlorophenoxyacetate (2, 4-D) and 6-Bensil Aminopurine (BAP) on MS Media on Texture and Color of Callus Explants of Teak Stem (Tectona grandis Linn. F. )"JUL". LanternBio: Biological Scientific Periodic. 2(1): 69-73.
Setiani A.N., Nurwinda F., and Astriany D. 2018. Effect of Disinfectants and Soaking Time on Sterilization of Breadfruit Leaf Explants (Artocarpus altilis (Parkinson ex. FA Zorn) Fosberg). Biotropics - Journal of Tropical Biology. 6(3): 78–82. https://doi.org/10.21776/ub.biotropica.2018.006.03.01
Sukweenadhi J, Choi JY, Kim YJ, Kaliraj L, Abid S, Ahn JC and Yang DC. Callus induction and in vitro mass culture of adventitious roots from leaf segment explants of Dendropanax morbifera Lev. InIOP Conference Series: Earth and Environmental Science 2019 Jun 1 (Vol. 293, No. 1, p. 012024). IOP Publishing.
Sumiati Y.E, and Lestiana A. 2014. Germination of Orchid seeds, Anthurium seeds, and Callus Induction of Binahong Leaf Explants. Plant Tissue Culture Practicum Report.
Suryowinoto M. 1996. In vitro Plant Breeding. Kanius, Yogyakarta.
Syahid S.F., Kristina N.N., and Seswita D. 2010. Effect of media composition on callus growth and tannin content of Dutch teak leaves (Guazuma ulmifolia Lamk) in vitro.
Turhan H. 2004.Callus induction and growth in transgenic potato genotypes. African Journal of Biotechnology. 3(8): 375-378.
Wahyuningrum ea. 2016. Screening of Potential Free Radicals Scavanger and Antibacterial Activities of Purwoceng (Pimpinella alpinaMolk). Tropical Life Sciences. 161-166.
Wardani D.P, Solichatun S., and Setyawan A.D.W.I. 2004. Growth and saponin production of Talinum paniculatum Gaertn. callus culture on various additions with 2, 4-dichlorophenoxy acetic acid (2, 4-D) and kinetin. Asian Journal of Natural Product Biochemistry. 2(1): 35-43.
Wetter L.R da, and Constable F. 1991. Methods of plant tissue culture, Second Edition. ITB Publisher, Bandung. 1-13.
Wickremisihe E.R.M., and Arteea, R.N. 1993. Taxus callus cultures: initiation, growth optimization, characterization and taxol production. Plant Cell, Tissue and Organ Culture. 35(2): 181-193.
Widodo P., Proklamasiningsih E., Sudiana E., Yani E., Budisantoso I., and Herawati W. 2018. Distribution of Purwoceng (Pimpinella Pruatjan Molkenb.) Past and Present. Seminar Proceedings National dan Call for Papers. (November): 376-383.
Widyawati G. 2010. Effect of variations in NAA and BAP concentrations on callus induction of Jatropha (Jatropha Curcas L.). UNS (Sebelas Maret University).
Zaerr J.B, and Mapes M.O. Action of growth regulators; 231–255 in Tissue culture in forestry, Springer.
Published
2022-12-16
How to Cite
Faramayuda, F., Irwan, M., & Syam, A. (2022). THE GROWTH OF PIMPINELLA ALPINA HOST CALLUS AT VARIOUS TREATMENTS OF PLANT GROWTH REGULATOR CONCENTRATIONS OF NAA. 2,4 D AND ITS COMBINATION WITH BAP. Agric, 34(2), 171-182. https://doi.org/10.24246/agric.2022.v34.i2.p171-182
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This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
