INDUKSI KALUS DAN PROLIFERASI ARTEMISIA CINA BERG EX POLJAKOV
DOI:
https://doi.org/10.24246/agric.2014.v26.i1.p45-51Keywords:
kondisi gelap, kondisi terang, proliferasi kalus, induksi kalus, Artemisia cinaAbstract
Penelitian ini bertujuan untuk mengevaluasi efek kondisi terang dan gelap pada induksi dan proliferasi kalus dari kalus Artemisia cina. Eksplan steril akan dikulturkan pada media MS dengan kandungan 2.4-D 1 mg/L dan diletakkan pada kondisi terang dan gelap. Hasil penelitian menunjukkan bahwa induksi kalus akan lebih efektif pada kondisi gelap, sedangkan proliferasi kalus lebih efektif pada kondisi terang. Tahap terbaik untuk proses regenerasi adalah hari ke-48 setelah transfer dari media induksi.
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References
Ahmad F, Khan RA, Rasheed S.1992. Study of analgesic and anti-Inflammatory activity from plant extracts of Lactuca scariola and Artemisia Absinthium. J. Islam. Acad. Sci., 5(2): 111-114
.
Armaka, M., E. Papanikolaou, A. Sivropoulou. 1999. Antiviral properties of isoborneol, a potent inhibitor of herpes simplex virus type 1. Antiviral Research 43: 79-92.
Basso, L.A., L.H. da Silva, A.G. Fett-Neto, W.F. de Azevedo Jr., I.S. de Moreira, M.S. Palma, J.B. Calixto, S. Astolfi-Filho, R.R. dos Santos, M.B. Soares, and D.S. Santos. 2005. The use of biodiversity as source of new chemical entities against defined molecular targets for treatment of malaria, tuberculosis, and T-cell mediated diseases; A Review. Memio Instituto do Oswaldo da Cruz 100: 475-506.
Cornejo-Martin, M.J., A.M. Mingo-Castel, and E. Primo-Millo. 1979.Organ redifferentiation in rice callus: Effects of C2H4,CO2, and cytokinins.Z. Pflanzenphysiol. 94:117-123.
Cubukcu B, Bray DH, Warhurst DC, Mericli AH, Ozhatay N, Sariyar G. 1990. In vitro antimalarial activity of crude extracts and compounds from Artemisia abrotanum L. Phytother. Res., 4(5): 203 204.
Fraser, R.S., U.E. Loening, and M.M. Yeoman. 1967. Effect of light on cell division in plant tissue cultures. Nature (London) 215:873.
George EF, Sherrington PD. 1984. Plant propagation by tissue culture. Exegetics LTD, Westbury.
Hangarter, R. P. and Stasinopoulos, T. C. Repression of plant tissue culture growth by light is caused by photochemical change in the culture medium. Plant Sci, 79(2) 253-257. 1991.
Harvey, A. 2000. Strategies for discovering drugs from previously unexplored natural products. Drug Discovery Today 5 (7): 294- 300.
Hayashi, K., T. Hayashi, H. Otsuka, (dkk.) 1997. Antiviral activity of 5,6,7-trimethoxyflavone and its potentiation of the antiherpes activity of acyclovir. Journal of Antimicrobial Chemotherapy 39, 821-4.
Khanna, H.K. and S.K. Raina, 1998. Genotype x Culture Medium Interaction Effects onRegeneration Response of Three Indica Rice Cultivars. Plant Cell Tissue and Organ Culture, 52: 145-153.
Kovats N, Goloncser F, Acs A, Refaey M. 2010. Quantification of the antibacterial properties of Artemisia absinthium, Artemisia vulgaris, Chrysanthemum leucanthemum and Achillea millefolium using the Vibrio fischeri bacterial bioassay. Act. Bot. Hungar, 52(1): 137-144.
Kristianti.E.B.E dan Herawati.M.M. 2009.Uji Antibakteri dari Artemisia cina.UKSW, Salatiga.
Kristianti.E.B.E dan Herawati.M.M.2010. Minyak Asiri Artemisia Sebagai Anti Bakteri. UKSW, Salatiga. 51 Callus Induction and Proliferation of Artemisia Cina Berg Ex Poljakov (Maria Marina Herawati et.al)
Lin, Y.M., M.T. Flavin, R. Schure, D.E. Zembower, and G.-X. Zhao. 1999. Biflavanoids and derivatives there of as antiviral agents. United States Patent 5948918. September 7, 1999.
Mousavi.E.S, Behbahani.M, Hadavi.E, Miri.S.M. 2012. Callus induction and plant regeneration in Lisianthus (Eustoma grandiflorium). Trakia Journal of Sciencies, Vol.10.No.1. 2012.
Muse, R. 1989. Physiology and Biochemistry of Witches Broom Disease in coca (Theolorona cocoal.) ph.D Thesis. Univ. of Liverpool, England, U.K.
Noth, M.H. and W.O. Abel. 1971. Zur entwicklung haploider pflanzen aus unreifen mikrosporen verschiedener Nicotiana arten. Z Pflanzenszucht. 65:277-284.
Ouyong, J., Wang, X. Zhoo, B and Wang Y. 2003. Light intensity and spectral quality influencing the callus growth of Cistanene deserticola and biosynthesis of phenylethanoid glycosides. Plant Science, 165:39 653-661.
Salehi. H. Salehi. M and Ashiri.F. 2008. Some condition for the best callus induction in Common Bernudagrass (Cynodon dactylon (L) Pers. California Origin. American-Eurasian J.Agric and Environ. Sci.3 (3):409-413.
Shirin F, Hossain M, Kabir MF, Roy M, Sarker SR. 2007. Callus inducation and plant regeneration from intermodal and leaf explants of four potato cultivars. World J. Agric. Sci. 3:1-6.
Smith, P.M. 1976. The Chemotaxonomy of Plants. London: Edward Arnold.
Summart. J, Panichajakul. S, Prathepa. P and Thanonkeo. P. 2008. Callus induction and influence of culture condition and culture medium on growth of Thai Aromatic Rice, Khao Dawk Mali 105, cell culture. World applied Science Journal 5 (2):246-251.
Tariq KA, Chishti MZ, Ahmad F, Shawl AS.2009. Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes. Vet. Parasitol, 160: 83-88.
Thorpe TA. 1990. The current status of plant tissue culture. In: Plant tissue culture, applications and limitations. (Bhojwani, S.S. Ed.). Amsterdam Elsevier, pp. 1-3.
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