Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer
Keywords:
ornamental chilli, RAPD-PCR, Capsicum chinense
Abstract
There are many varieties of ornamental chilli that may different from their ancestor and cause genetic variability. This research aims to determine the relationship of ornamental chillies in Indonesia based on their fruit morphology and RAPD-PCR. Morphological was analysed by description method, while molecular was analysed by RAPD-PCR using OPA 12 primer. Result showed that Katty, Naga Morich, Big Black Mama, and Yellow Primo were Capsicum chinense based on their morphology and RAPD-PCR using OPA-12 primer.
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References
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Thorman, C.E., M.E. Ferreira, L.E.A. Camargo, J.G. Tivang, and T.C. Osborn. 1994. Comparison of RFLP and RAPD markers to estimating genetic relationship within and among cruciferous spesies. Theoritical and Applied Genetic 88: 973-980.
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Horejsi, T. and J.E. Staub. 1998. Genetic variation in cucumber (Cucumis sativus L) as assessed by random amplified polymorphic DNA. Abstrak TEKTRAN. Washington D.C.: United States Department of Agriculture, Agricultural Research Service, USA.
Perry, L., Dickau, R., Zarrillo, S., Holst, I., Pearsall, D.M., Piperno, D.R., Berman, M.J., Cooke, R.J., Rademaker, K., Ranere, A.J., Raymond, J.S., Sandweiss, D.H., Scaramelli, F., Tarble, K., and Zeidler, J.A. 2007. Starch Fossils and the Domestication and Dispersal of Chili Peppers (Capsicum spp. L.) in the Americas. Science 315 : 986 – 988.
Sikora, B. and Nowaczyk. 2014. Application of RAPD Technique for Identification of Interspecific Hybrids from Genus Capsicum. Acta Sci. Pol., Hortorum Cultus 13(1): 155-166
Thorman, C.E., M.E. Ferreira, L.E.A. Camargo, J.G. Tivang, and T.C. Osborn. 1994. Comparison of RFLP and RAPD markers to estimating genetic relationship within and among cruciferous spesies. Theoritical and Applied Genetic 88: 973-980.
Walsh, B.M. and Hoot, S.B. 2001. Phylogenetic Relationships OfCapsicum (Solanaceae) Using DNA Sequences From Two Noncoding Regions: The Chloroplast Atpb-Rbcl Spacer Region And Nuclear waxyIntrons. International Journal of Plant Science 162 : 1409–1418.
Waugh, R. and W. Powell. 1992. Using RAPD markers for crop improvement. TIBTECH. 10: 186-191
How to Cite
Ferniah, R., Kusdiyantini, E., & Nurhayati, N. (1). Phylogenetic of Ornamented Chilli Based on the Fruit Morphology and Molecular Marker using OPA-12 Primer. Agric, 30(1), 57-62. https://doi.org/10.24246/agric.2018.v30.i1.p57-62
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
