NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN
DOI:
https://doi.org/10.24246/agric.2018.v30.i1.p25-32Keywords:
Azotobacter, Damping off, NPK fertilizer, PGPR, YieldAbstract
Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
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References
Ahmad F, Ahmad, I., Khan M-S. 2008. Screening of Free-Living Rhizospheric Bacteria for Their Multiple Plant Growth Promoting Activities. Microbiology Research. 163 (2): 173—181
Bhosale H.J. Kadam T.A., Bobade A.R. 2013. Identification and Production of Azotobacter vinelandii and Its Antifungal Activity Against Fusarium oxysporum. Journal of Environmental Biology. 34 (2):177-182
El-Barougy, E, N-M Awad, A-S Turky, and H-A Hamed. 2009. Antagonistic Activity of Selected Strains of Rhizobacteria Against Macrophomia Phaseolina of Soybean Plants. American European Journal of Agriculture and Environment Science. 5 (3):337-347
Gauri S-S, Mandal, S-M., and Pati B.R. 2012. Impact of Azotobacter Exoplysaccharides on Sustainable Agriculture. Applied Microbiology Biotechnology. 95 (2): 331 – 338
Glick B.R. 2012. Plant Growth-Promoting Bacteria: Mechanisms and Applications. Hindawi Publishing Corporation. Scientifica. Vol. 2012, Article ID 963401, pp15.
Hindersah R., Setiawati M.R., Fitriatin B.N., Suryatama P., Asmiran, P., Panatarani C., Joni I.M. 2018. Graphite tail powder and liquid biofertilizer as trace elements source for ground nut. Hal 030004-1 - 030004-8. Dalam AIP Conference Proceedings, The 1st International Conference and Exhibition on Powder Technology Indonesia, Jatinangor. 8-9 Agustus 2017
Kader M.A., Mian M.H., Hoque M.S. 2002. Effect of Azotobacter Inoculant on Yield and Nitrogen Uptake by Wheat. Journal Biology Science. 2 (4):25 -251.
Mali G.V., Bodhankar M.G. 2009. Antifungal and Phytohormone Production Potential of Azotobacter chroococcum Isolates from Groundnut (Arachis hypogea L.) Rhizospere. Research Journal of Chemistry and Environment. 15 (2):1-7
Nosrati R., Owlia P., Saderi H., Rasooli I., Ali Malboobi M. 2014. Phosphate Solubilization Characteristics of Efficient Nitrogen Fixing Soil Azotobacter Strains. Iranian Journal of Microbiology. 6 (4):285-295
Ponmurugan K.A., Sankaranarayanan, Al-Dharbi N.A. 2012. Biological Activities of Plant Growth Promoting Azotobacter sp. Isolated from Vegetable Crops Rhizosphere Soils. Journal of Pure and Applied Microbiology. 6 (4):147-154
Posada L.F., Ramírez M., Ochoa-Gómez N., Cuellar-Gaviria T.Z., Argel-Roldan L.E., Ramírez C-A., Villegas-Escobar V. 2016. Bioprospecting of Aerobic Endospore-Forming Bacteria with Biotechnological Potential for Growth Promotion of Banana Plant. Scientia Horti. 212: 81–90
Sattar M.A., Rahman M.F., Das D.K., Choudhury T.M.A. 2008. Propects of using Azotobacter, Azospirillum and cyanobacteria as supplements of urea nitrogen for rice production in Bangladesh. http://www.aciar.gov.au/system/files/node/9817/ pr130+part+3.pdf. [24/12/ 2008].
Tilak K.V.B.R., Ranganayak N., Manoharachar, C. 2006. Synergistic Effects of Plant-Growth Promoting Rhizobacteria and Rhizobium on Nodulation and Nitrogen Fixation by Pigeonpea (Cajanus cajan). European Journal of Soil Science. 57 (1): 67-71
Verma S., Kumar V., Narula N, Merbach W. 2001. Studies in Vitro Production of Antimicrobial Substances by Azotobacter chroococcum Isolates/Mutans. Journal of Plant Diseases Protection. 108(2):152-165.
Vikhe P.S. 2014. Azotobacter species as a Natural Plant Hormone Synthesizer. Research Journal on Recent Science. 3 (IVC):59-63.
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