Komposisi Kimia Penyusun Minyak Atsiri Daun Insulin (Tithonia diversifolia (Hamsley) A.Gray) dari Kalimantan Selatan
DOI:
https://doi.org/10.24246/juses.v3i2p32-37Keywords:
daun insulin, distilasi, limonen, minyak atsiri, Tithonia diversifoliaAbstract
Daun Insulin (Tithonia diversifolia) merupakan salah satu tanaman obat tradisional yang digunakan masyarakat Kalimantan Selatan untuk mengatasi glikemia. Analisis komposisi Kimia penyusun minyak atsiri dari daun Insulin yang berasal dari Kalimantan Selatan belum dilakukan. Oleh karena itu, penelitian ini bertujuan untuk menentukan komponen kimia penyusun minyak atsiri Daun Insulin (T. diversifolia) untuk melengkapi data dasar daun Insulin yang diperoleh dari Kalimantan Selatan. Sampel daun Insulin diperoleh tumbuh liar dari Desa Hulu Pasar, Kabupaten Hulu Sungai Utara Kalimantan Selatan, Indonesia. Sebelum didistilasi, daun dikeringanginkan kemudian dikeringkan dengan oven pada suhu 50 ᵒC selama 30 menit. Metode distilasi rebus digunakan untuk mendapatkan minyak atsiri daun Insulin. Sampel didistilasi selama 48 jam sehingga diperoleh minyak bening berwarna kekuningan dengan rendemen minyak 0,02% (b/b). Minyak hasil distilasi selanjutnya dianalisis dengan Kromatografi Gas-Spektra Masa (KG-SM). Hasil kromatogram minyak atsiri daun Insulin menunjukkan adanya 6 puncak yang teridentifikasi melalui spektra massa merupakan senyawa limonen (32,14%), β pinen (24,88%), α pinen (21,73%), trans kariofilen (11,68%), bisiklogermakren (5,24%), dan β felandren (4,33%). Komponen kimia penyusun minyak atsiri daun Insulin termasuk dalam golongan monoterpena dan seskuiterpena hidrokarbon.
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