Isolation of Bacteriophages and Their Application to Control Pathogenic Bacteria
Bakteriofag untuk Mengontrol Bakteri Patogen
DOI:
https://doi.org/10.24246/tmj.v1i1.10658Keywords:
Bacteriophage, Foodborne disease, Food safety, PathogensAbstract
Foodborne disease (FBD) is a phenomenon of food poisoning caused by pathogenic bacteria. Diseases caused by FBD can be in the form of mild diseases such as diarrhea or dangerous diseases that can lead to death. An alternative effort to overcome the phenomenon of FBD is by using antibiotics as a pathogenic bacterial control agent. The negative impact of using antibiotics can evoke pathogenic bacteria's ability to resist antibiotics. The use of bacteriophages specific to pathogenic bacteria has become one of the alternatives to eliminate the use of antibiotics. This research aims to obtain bacteriophages that can inhibit pathogenic bacteria. Bacteriophage isolation was carried out from several samples: wastewater, fresh chicken intestines, salted vegetables, and soil. The results showed that the isolation of bacteriophages produced virulent mixed bacteriophages from chicken intestinal samples with isolate number U14 (Փ U14). Փ U14 can lyse all of the host bacteria in the addition of 30 minutes, 60, 90, and 120. Փ U14 with a concentration of 1.05 x 108 PFU / mL effectively inhibits the growth of pathogenic bacteria such as Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Bacilluscereus but cannot inhibit growth Escherichia coli. Identification of the host bacterium U14 showed that the bacterium was Gram-negative and catalase-positive.
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