ANALYSIS OF SECONDARY METABOLITES OF CALLUS OF RAMBUTAN Nephelium lappaceum L

  • Fahrauk Faramayuda Faculty of Pharmacy Universitas Jenderal Achmad Yani
  • Elfahmi Elfahmi School of Pharmacy, Bandung Institute of Technology (ITB), Bandung, West Java, Indonesia, 40132.
  • Weni Widy Astuti Faculty of Pharmacy, Jenderal Achmad Yani University (UNJANI), Cimahi, West Java, Indonesia, 40532
Keywords: rambutan plants, plant tissue culture, hormonal growth, thin layer chromatography

Abstract

Rambutan plant (Nephelium lappaceum L.) is a member of the Sapindaceae family. The rambutan plant is one of the natural ingredients that can be developed as traditional medicine. Rambutan peel has the potential for good antioxidant and anticancer activity. Rambutan fruit does not grow every time it needs efforts to produce the active substance in rambutan, using plant tissue culture techniques. The use of the correct variety of mediums and hormones at the right concentration is the key to thriving tissue culture. Explants derived from rambutan leaves were planted precisely on solid media Murashige and Skoog (MS) and WoddyPlant Medium (WPM) containing Indole-3-Butyric Acid (IBA) and Kinetin. After seven days, the callus was subcultured, then after 35 days, the subculture callus was collected and dried. Dry callus and rambutan leaves (Wild type) were macerated with n-hexane, ethyl acetate, and ethanol. The concentrated extract was then applied to a GF 254 silica gel plate with the mobile phase Toluene-Acetone (7: 3) and n-hexane-EthylAsetate (3: 7). The results showed that the concentration of IBA 2 ppm and kinetin three ppm was the best combination because it produced callus. TLC results of rambutan leave with plant tissue culture containing flavonoids and triterpenoids. This study provides new information regarding the induction of rambutan callus and can become the basis for producing active metabolites in rambutan with cell suspension culture development.

 

Downloads

Download data is not yet available.

References

Abidin, Zainal. 1993. Knowledge Basics About Growing Regulatory Substances. Bandung: Space. p. 1, 44.

Chen HS, Bai MH, Zhang T, Li GD, Liu M. 2015. Ellagic acid induces cell cycle arrestand apoptosis through TGF-β / Smad3 signaling pathway in human breast cancer MCF-7cells, Int J Oncol.; 46 (4): 1730-8.

Cronquist, A. 1981. An Integrated System of Clasification of Flowering Plants. New York: Columbia University Press. Page. 795-797.

Dalimarta, S. 2003. Atlas of Indonesian Medicinal Plants, volume 3. Jakarta: Puspa Swara. Page. 116.

Gambari R, Hau DK, Wong WY, Chui CH, 2014.Sensitization of Hep3B hepatoma cellsto cisplatin and doxorubicin by corilagin. Phytother Res, 28 (5): 781-3.

Hariana, A., 2006. Medicinal Plants and Their Benefits, Series 3, Penebar Swadaya, Jakarta.

Hendaryono, Sriyanti P Daisy, Ari Wijayani. 1994. Tissue Culture Techniques; Introduction and Vegetative Modern Plant propagation instructions. Yogyakarta: Kanisius. Hal. 26-28, 17.

Jia L, Jin H, Zhou J, Chen L, Lu Y, Ming Y, Yu Y. 2013. A potential herbal anti- tumormedicine, Corilagin, ovarian cancer cell growth through blocking the TGF-βsignaling pathways. BMC Complement Altern Med.

Kashiwada Y, Nonaka G, Nishioka I, Chang JJ, Lee KH. 1992. Tannins and related compounds as selective cytotoxic agents. Antitumor agents, J Nat Prod; 55 (8): 1033-43.

Li J, Wang S, Yin J, Pan L. 2013. Geraniin induces apoptotic cell death in human lungadenocarcinoma A549 cells in vitro and in vivo. Can J Physiol Pharmacol; 91 (12): 1016-24.

Losso JN, Bansode RR, TrappeyA2nd, Bawadi HA, Truax R. 2004 Nov. In vitro anti- proliferativeactivities of ellagic acid. J Nutr Biochem ; 15 (11): 672-8.

Markham, K.R.1982. Cara Mengidentifikasi Flavonoid, diterjemahkan oleh Kosasih Padmawinata. Bandung: Penerbit ITB.

Okabe S, Suganuma M, Imayoshi Y, Taniguchi S, Yoshida T, Fujiki H. 2001. New TNFalphareleasing inhibitors, geraniin and corilagin, in leaves of Acer nikoense, Megusurino-ki. Biol Pharm Bull. t; 24 (10): 1145-8.

Pardal, S. J., Ika, M., E. G. Lestari., dan Slamet. 2004. Regenerasi Tanaman dan Transformasi Genetik Salak Pondoh untuk Rekayasa Buah partenokarpi. J. Bioteknologi Pertanian.

Pierik, R.L.M. 1997. In vitro Culture of Higher Plants. The Netherlands: Kluwer Academic Publisher, Dordrect. p. 90-91.

Ria, Johny. 1994. Indonesian Medicinal Plant Inventory (III). Jakarta: AgencyResearch and Health movement phases.

Setiti, E.W.U., Sri Puji A.W. and T. Sudarti. 1996. The role of the media and ZPT forcallus induction and differentiation in melon tissue cultivation. Journal Horticulture.

Smith, R.H. 1992. Plant Tissue Culture: Technique and Experiments. New York: Academic Press Inc.

Strati A, Papoutsi Z, Lianidou E, Moutsatsou P. 2009. Effect of ellagic acid on theexpression of human telomerase reverse transcriptas (hTERT) alpha + beta + transcript inestrogen receptor- positive MCF-7 breast cancer cells. Clin Biochem ; 42 (13-14): 1358-62.

Thitilertdecha, N, Teerawutgulrag,A, Jeremy D. Kilburn and Rakariyatham. N. 2010. Identification of Major Phenolic Compounds from Nephelium lappaceum L. and TheirAntioxidant Activities, Molecules, 15, 1453-1465.

Wagner H., Bladt S., Zgainski E.M. 1984. Plant Drug Analysis: A Thin Layer Chromatography Atlas. New York: Springer-Verlag.

Wetherell, D. F. 1982. Pengantar Propagasi Tanaman secara In Vitro Seri Kultur Jaringan Tanaman. New Jersey: Avery Publishing Group.

Wetter, L. R., dan F. Constabel. 1991. Metode Kultur Jaringan Tanaman. Bandung: ITB.

Zhang T, Chen HS, Wang LF, Bai MH, Wang YC, Jiang XF, Liu M. 2014. Ellagic acidAnti-proliferation effects exerts via modulation of Tgf-β / Smad3 signaling in MCF-7breast cancer cells. Asian Pac J Cancer Prev. (1): 273-6.

Zulkarnain. 2009. Plant Tissue Culture. Jakarta: Bumi Aksara. Hal. 38-40, 93-94, 111, 112, 98-99, 100-101,161-162.
Published
2021-08-01
How to Cite
Faramayuda, F., Elfahmi, E., & Astuti, W. (2021). ANALYSIS OF SECONDARY METABOLITES OF CALLUS OF RAMBUTAN Nephelium lappaceum L. Agric, 33(1), 13-22. https://doi.org/10.24246/agric.2021.v33.i1.p13-22
Section
Articles